Centrifugation Sedimentation Coefficient
Sedimentation coefficient (S) is a measure of how quickly a particle settles during centrifugation. It tells us how fast a particle moves under the force of gravity applied during centrifugation. It is represented by the symbol “S” and is measured in seconds. A typical value for sedimentation coefficient is 1 × 10-13 sec.
To find sedimentation coefficient, scientists measure how the concentration of a substance changes over distance as it settles during centrifugation.
Sedimentation coefficient depends on the size, mass, and density of the particle. It is directly related to the particle’s buoyant molar mass (Mb) and inversely related to its frictional coefficient (f).
In simpler terms, sedimentation coefficient helps us understand how quickly particles of different sizes, masses, and densities settle in a centrifuge.
Centrifugation
Centrifugation is a technique used to separate components of a mixture based on their size, shape, viscosity and density. It works by spinning the mixture at high speed in a machine called a centrifuge, which creates a force called centrifugal force. This force pushes denser components outward, away from the centre of the centrifuge, while less dense components are pushed inward.
Key Points:
- What it does: Separates components in a mixture.
- How it works: Uses centrifugal force created by spinning the mixture at high speed.
- What separates: Based on size, shape, and density.
- Used in: Many fields like biology, chemistry, medicine, and industry.
Learn about, the churning process, centrifugation process, types of centrifugation, and others in detail in this Centrifugation Notes.
Table of Content
- What is Centrifugation?
- Centrifugation Diagram
- Factors Affecting Centrifugation
- Principle of Centrifugation
- Centrifugation Types
- Application of Centrifugation