Conclusion – Differences Between SDS PAGE and Native PAGE
In conclusion, SDS-PAGE and Native PAGE are two distinct techniques in molecular biology for protein separation. This article discusses the comparision between SDS PAGE and Native PAGE. SDS-PAGE separates proteins by molecular weight in denaturing conditions. Native PAGE separates proteins based on size and charge under non-denaturing conditions. It also preserves native conformation and functionality of the proteins. SDS-PAGE is used for protein detection and molecular weight determination. Native PAGE is used for studying protein structure, composition, and function.
Differences between SDS PAGE and Native PAGE
The difference between SDS-PAGE and native PAGE lies in how proteins are separated in the polyacrylamide gel. In SDS-PAGE, the migration rate of the protein is determined by its molecular weight only, whereas in native PAGE, the migration rate depends on both the size and overall charge of the protein. In this article, we will study the process of SDS PAGE and Native PAGE as well as the similarities and differences between SDS-PAGE and native PAGE.
Table of Content
- Differences Between SDS PAGE and Native PAGE
- What is SDS PAGE?
- What is Native PAGE?
- Similarities between SDS PAGE and Native PAGE
- Conclusion – Differences Between SDS PAGE and Native PAGE
- FAQs on SDS PAGE and Native PAGE