Cryopreservation Procedure
Cryopreservation is done carefully and involves steps that prevent cell death while arresting its metabolic activities. This is typically done by the use of dry ice or liquid nitrogen. The steps involved in Cryopreservation are discussed below.
- Selection of material: Select the material based on factors such as size, shape, mass, and acidity to ensure that the biological materials are not damaged.
- Addition of cryoprotectants: Glycerol, FBS, salts, sugars, and glycols are added to cryo-protected substances. This helps to lower the freezing point and slow down the cooling process and prevents the risk of formation of ice crystals.
- Freezing: Various freezing methods protect cells from damage and prevent death by exposing them to warm solutions of Cryogenic Protective Agents.
- Storage in Cold Conditions: Keep the cryopreserved samples in extremely cold places, like -80°C in a freezer, for at least 5 to 24 hours before moving them to storage containers.
- Thawing: Warm the biological samples when necessary to control the cooling rate and avoid cell damage from crystal formation.
Cryopreservation
Cryopreservation is a method to preserve cells and tissues for longer periods at the freezing points typically at -196 degrees Celsius. However, this cooling is carried out carefully and involves the use of cryoprotectants that prevent the crystallization of cellular materials. In this article, we will learn about Cryopreservation, its procedure, methods, and applications.
Table of Content
- What is Cryopreservation?
- Cryopreservation Procedure
- Diagram of Cryopreservation
- Different Methods of Cryopreservation
- Usage of Cryopreservation
- Benefits of Cryopreservation
- Applications of Cryopreservation
- Advantages and Disadvantages of Cryopreservation