Importance of SDS-PAGE
In molecular biology and biochemistry, sodium dodecyl sulphate polyacrylamide gel electrophoresis, or SDS-PAGE, is a crucial and often applied method.
- Seperation of Protein: Proteins in a sample can be effectively separated using SDS-PAGE. It gives scientists a visual depiction of the protein composition and enables them to identify specific proteins within a complicated combination.
- MW determination: SDS-PAGE allows the molecular weight of unknown proteins to be estimated by running known molecular weight standards alongside the sample.
- Purity analysis: Protein samples may be tested for purity using SDS-PAGE. Impurities can be identified by contaminants or extra bands in the gel, which enables researchers to assess how well protein purification techniques worked.
- Quantification of Proteins: Protein levels can be semi-quantitatively analysed since the intensity of the protein bands on the gel correlates with protein concentration.
- Western Blotting: Western blotting is a technique that uses antibodies to identify and quantify certain proteins.
SDS-PAGE
SDS PAGE, or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, is a technique for separating proteins depending on their molecular weight. SDS is an anionic surfactant and detergent. SDS breaks down the non-covalent links of protein molecules. The method of separating protein molecules according to their electrophoretic mobility is frequently used in molecular biology, genetics, forensics, and biotechnology. In this article, we will read about SDS-PAGE, its principles, its methods, and procedures required to carry out its process, and its applications.
Table of Content
- Define SDS-PAGE
- What is Electrophoresis?
- What is SDS-PAGE?
- Principle of SDS-PAGE
- Materials Required
- Procedure of SDS-PAGE
- Applications of SDS-PAGE
- Importance of SDS-PAGE