Limitation of Benedict’s Test
Limitation of Benedict’s Test are,
- Certain medications, such as salicylates, isoniazid, streptomycin, penicillin, and p-aminosalicylic acid, might potentially result in false-positive test results.
- Benedict’s reaction may be slightly lessened by compounds (urate, creatinine, and ascorbic acid) in concentrated urine.
- To display precise concentration of reducing sugar, only an estimated semi-quantitative number can be utilized.
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Benedict’s Test
Benedict’s Test is a chemical test used to identify reducing sugars present in the solution. Reducing sugars are those sugars that have reducing properties. All monosaccharides and disaccharides (apart from sucrose) are reducing sugars, such as glucose, fructose, lactose, etc; they contain an aldehyde (-CHO) or a ketonic C = O. Benedict’s Test also called Benedict’s Solution or Benedict’s Reagent is used to identify simple sugars like glucose.
In this article, we will learn about Benedict’s Test, Procedure of Benedict’s Test, Preparation of Benedict’s Reagent, and others in detail.
Table of Content
- What is Benedict’s Test?
- Principle of Benedict Test
- Preparation of Benedict’s Reagent
- Procedure of Benedict’s Test
- Testing for Reducing Sugars
- Applications of Benedict’s Test
- Limitation of Benedict’s Test