Sandwich ELISA
- It helps to detect antigens in samples.
- Here microtiter well is coated with antibodies.
- Add a sample that contains antigen to the wells and wash it to remove the free antigen.
- Now an enzyme-bound secondary antibody that binds to a different epitope on the antigen is added. Wash the wells to remove all free secondary antibodies.
- An enzyme-specific substrate is added to the plate to form a measurable color product.
What is ELISA? – Introduction, Procedure, Types, Applications
A basic array technique called enzyme-linked immunosorbent assay that basically used to identify and measure the antibodies, proteins, peptides, and hormones in the blood. Its test result can provide us the information about the disease that may help in planning treatment. It compares with other antibody assays to provide quantitative results and separation of non-specific and specific interactions caused by continuous binding to solid surfaces, usually polystyrene multiwell plates.
Antigen (Ag)
A toxic molecule or any foreign matter that causes an immune response in the body is called an antigen. In immunology, an antigen term originally referred to substances that generate antibodies. Antigens can be proteins, peptides, lipids, or nucleic acids.
Antibody (Ab)
An antibody is a blood protein that is produced in our body by the immune system in response to and to counteract a specific antigen. Antibodies are also called immunoglobulins.