Some Common Challenges Faced with Plant Tissue Culture
It is a lot of work to put in for running an effective in vitro culture, hence requires more labor and space that may cost more than you’ve budgeted. Along with this issue, there are a few common challenges you may face, like:
- Inadequate selection of nutrient medium, causing hormonal imbalance.
- Contamination is the most common problem in tissue culture planting including bacteria, fungi, and yeast.
- A right-constructed laboratory with a washing room, appropriate sterilization, and growth room is a must for successful cultures and reducing contamination sources.
- Right usage of antibiotics helps in enhancing the growth of the plant tissue like Streptomycin, Rifampicin, and Cefotaxime.
- The release of Oxidized stress of phenol may lead to browning of explants that reduces the cell division pace and capacity of regeneration.
- Concentrated somaclonal variations may influence genetic variability.
Tissue Culture Plants
Over the centuries, farms and cultivation fields are feeding everyone on the planet. However, in the last few decades, with the increasing population and colonization, the shrinking of land is making it difficult to enough plant food along with challenging weather conditions.
Hence, Tissue culture was introduced as an alternative cultivation method compound with conventional techniques to develop a large number of genetically identified plants from one single plant, called the parent plant. It is extensively used for the mass production of disease-free commercial plants.