Types of ELISA
There are three types of ELISA tests that are distinguished on the basis of methods used for binding between antibodies and antigens-
- Indirect ELISA
- Sandwich ELISA
- Competitive ELISA
What is ELISA? – Introduction, Procedure, Types, Applications
A basic array technique called enzyme-linked immunosorbent assay that basically used to identify and measure the antibodies, proteins, peptides, and hormones in the blood. Its test result can provide us the information about the disease that may help in planning treatment. It compares with other antibody assays to provide quantitative results and separation of non-specific and specific interactions caused by continuous binding to solid surfaces, usually polystyrene multiwell plates.
Antigen (Ag)
A toxic molecule or any foreign matter that causes an immune response in the body is called an antigen. In immunology, an antigen term originally referred to substances that generate antibodies. Antigens can be proteins, peptides, lipids, or nucleic acids.
Antibody (Ab)
An antibody is a blood protein that is produced in our body by the immune system in response to and to counteract a specific antigen. Antibodies are also called immunoglobulins.